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Provedor de dados:  Nature Precedings
País:  United Kingdom
Título:  "Dark Mediators" of Proteins as Revealed by NMR in Water: Residue-selective Anion Bindings that are Masked by Pre-existing Buffer
Autores:  Jianxing Song
Linlin Miao
Haina Qin
Data:  2012-01-10
Ano:  2012
Palavras-chave:  Chemistry
Molecular Cell Biology
Earth & Environment
Evolutionary Biology
Resumo:  Ions are commonly believed to impose their effects on proteins by unspecific electrostatic screening. Here, by NMR we reveal that in water sulfate, chloride and thiocyanate are able to bind a well-folded WW domain at distinctive residues and affinities, which is surprisingly masked by the pre-existing buffer. Our study reveals that the specific anion binding is so ubiquitous and consequently no longer negligible in establishing "postreductionist framework" for protein biochemistry.
Tipo:  Manuscript
Identificador:  http://precedings.nature.com/documents/6769/version/1

oai:nature.com:10101/npre.2012.6769.1

http://hdl.handle.net/10101/npre.2012.6769.1
Fonte:  Nature Precedings
Direitos:  Creative Commons Attribution 3.0 License
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