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Provedor de dados:  Inra
País:  France
Título:  Construction of a cytogenetically anchored microsatellite map in rabbit
Autores:  Chantry-Darmon, C.
Urien, C.
Hayes, H.
Bertaud, M.
Chadi, S.
Chardon, P.
Vaiman, D.
Rogel-Gaillard, C.
Data:  2005
Ano:  2005
Palavras-chave:  CARTE GENETIQUE
Resumo:  Rabbit (Oryctolagus cuniculus) represents a valuablesource of biomedical models and corresponds to asmall but active economic sector in Europe for meatand fur. The rabbit genome has not been thoroughlystudied until recently, and high-resolution mapsnecessary for identification of genes and quantitativetrait loci (QTL) are not yet available. Our aim was toisolate over 300 new and regularly distributed (TG)nor (TC)n rabbit microsatellites. To achieve thispurpose, 164 microsatellite sequences were isolatedfrom gene-containing bacterial artificial chromosome(BAC) clones previously localized by fluorescencein situ hybridization (FISH) on all the rabbitchromosomes. In addition, 141 microsatellite sequenceswere subcloned from a plasmid genomiclibrary, and for 41 of these sequences, BAC cloneswere identified and FISH-mapped. TC repeats werepresent in 62% of the microsatellites derived fromgene-containing BAC clones and in 22% of thosefrom the plasmid genomic library, with an average of42.9% irrespective of the microsatellite origin. Theseresults suggest a higher proportion of (TC)n repeatsand a nonhomogeneous distribution of (TG)n and(TC)n repeats in the rabbit genome compared tothose in man. Among the 305 isolated microsatellites,177 were assigned to 139 different cytogeneticpositions on all the chromosomes except rabbitChromosome 21. Sequence similarity searches providedhit locations on the Human Build 35a andhypothetical assignments on rabbit chromosomes for ten additional microsatellites. Taken together,these results report a reservoir of 305 new rabbitmicrosatellites of which 60% have a cytogeneticposition. This is the first step toward the constructionof an integrated cytogenetic and genetic mapbased on microsatellites homogeneously anchored tothe rabbit genome.
Tipo:  Journal Article
Idioma:  Inglês
Formato:  application/pdf
Fonte:  Mammalian Genome. 2005, 16 : 442-459

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