| Registro completo |
| Provedor de dados: |
OAK
|
| País: |
Japan
|
| Título: |
Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
|
| Autores: |
Tagawa, Michihito
Yamamoto, Yuhei
Shimbo, Genya
Iguchi, Aiko
Xuan, Xuenan
Tomihari, Mizuki
Miyahara, Kazuro
|
| Data: |
2017-05
|
| Ano: |
2017
|
| Palavras-chave: |
CD152
CTLA-4
Dog
Soluble form
Splicing variant
|
| Resumo: |
Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study.
|
| Idioma: |
Inglês
|
| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/4517
info:doi/10.1292/jvms.16-0583
|
| Editor: |
Japanese Society of Veterinary Science
|
| Direitos: |
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-ncnd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
|
