| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential
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| Autores: |
Dautu, George
Munyaka, Biscah
Carmen, Gabriella
Zhang, Guohong
Omata, Yoshitaka
Xuan, Xuenan
Igarashi, Makoto
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| Data: |
2007-07
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| Ano: |
2007
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| Palavras-chave: |
Toxoplasma gondii
DNA
Deoxyribonucleic acid
DNA immunization
MIC2
Microneme protein-2
M2AP
MIC2 associated protein
AMA1
Apical membrane antigen-1
BAG1
Bradyzoite antigen-1
IgG
Immunoglobulin G
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| Resumo: |
A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-γ synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes.
http://www.sciencedirect.com/science/journal/00144894
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/1031
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| Editor: |
Elsevier
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| Formato: |
application/pdf
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