| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Determination of Prostaglandins in the Colostrum of Dairy Cattle by Thermospray High-Performance Liquid Chromatography-Mass Spectrometry.
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| Autores: |
FUKUSHIMA, Michihiro
NAKANO, Masuo
ENDO, Tsuyoshi
NEGISHI, Takashi
福島, 道広
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| Data: |
1993
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| Ano: |
1993
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| Palavras-chave: |
Thermospray liquid chromatography-mass spectrometry
Prostaglandin
Colostrum
Dairy cattle
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| Resumo: |
A thermospray high-performance liquid chromatography-mass spectrometry (TSP HPLC-MS) method is described for the analysis of prostaglandins (PGs) E1 and E2, which have several physiological actions at the trace level. HPLC separation was carried out on a reversed-phase column by using 50mM ammonium acetate (pH 4.5)-acetonitrile (7:3, v/v) as the mobile phase at a flow rate of 1.0ml/min. In MS detection, the vaporizer temperature was set at 220°C and the ion-source block temperature at 264°C. The mass spectra obtained from PGs typically exhibited the [M+NH4]+ ion (5 to 30% relative intensity) and several fragment ions due to sequential losses of water from both the [M+H]+ and [M+NH4]+ ions. PGE1 (46.9ppb) and PGE2 (1.9ppb) in the colostrum of dairy cattle were identified and quantitated. The PGE1 concentration in the colostrum and mature milk of dairy cattle showed a similar pattern to that in humans.
https://www.jstage.jst.go.jp/article/analsci1985/9/5/9_5_631/_article/-char/ja/
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/4042
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| Editor: |
日本分析化学会
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| Direitos: |
日本分析化学会
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