| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Synthesis of tag introducible (3-trifluoromethyl)phenyldiazirine based photoreactive phenylalanine
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| Autores: |
Hashimoto, Makoto
Hatanaka, Yasumaru
Sadakane, Yutaka
Nabeta, Kensuke
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| Data: |
2002-09-16
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| Ano: |
2002
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| Palavras-chave: |
FLAVIN BINDING-SITES
PHOTOAFFINITY
DIAZIRINE
IDENTIFICATION
PROTEINS
RECEPTOR
DOMAIN
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| Resumo: |
An efficient synthesis of tag introducible (3-trifluoromethyl)phenyldiazirine based phenylalanine derivatives is described. Alkylation of a chiral glycine equivalent with a spacer containing (3-trifluoromethyl)phenyldiazirinyl bromides enables us to create photoreactive L-phenylalanine derivatives. After introduction of biotin at the spacer, the biotinylated and photoreactive amino acid was applied for L-amino acid oxidase and incorporated into a substrate binding site. These compounds will be powerful tools not only for photoaffinity labeling to elucidate properties of bioactive peptides but also as trifunctional photophors to introduce a ligand skeleton. (C) 2002 Elsevier Science Ltd. All rights reserved.
http://www.sciencedirect.com/science/journal/0960894X
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/713
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| Editor: |
PERGAMON-ELSEVIER SCIENCE LTD
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| Formato: |
application/pdf
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