| Registro completo |
| Provedor de dados: |
Animal Sciences
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| País: |
Brazil
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| Título: |
Colorimetric method as alternative to chromium (III) quantification in cattle feces
Colorimetric method as alternative to chromium (III) quantification in cattle feces
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| Autores: |
Torres Ospina, Alexandra
Zapata, Julian Andrés
Gil Gonzalez, Jesus Humberto
Giraldo, Luis
Valencia, Diana Marcela
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| Data: |
2020-04-01
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| Ano: |
2020
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| Palavras-chave: |
Spectrophotometry
Fecal output
External marker
Chromium dioxide
Validation. spectrophotometry
Fecal output
External marker
Chromium dioxide
Validation.
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| Resumo: |
One of the main factors to establish productivity of grazing cattle is the estimation of forage intake. For this, the most widely used technique is based on the estimation of fecal output using chromium dioxide as external marker. However, quantification can be expensive and sometimes not precise due to the methodology used for this purpose. Therefore, the aim of this study was to validate the colorimetric method for chromium quantification and to implement it in the estimation of fecal output in grazing cattle. The temperature, the digestion time and the wavelength for the measurement were evaluated. The method was validated for selectivity, linearity, detection and quantification limits, precision, accuracy, and stability. Results showed that temperature and digestion time are critical to improve sensitivity and quantification limits. The validation demonstrated that the method is suitable for the quantification of Cr2O3 in a wide range of concentrations, being statistically comparable with a reference method, and offering a reliable low cost and easy to implement alternative, to estimate fecal output in bovine digestibility studies.
One of the main factors to establish productivity of grazing cattle is the estimation of forage intake. For this, the most widely used technique is based on the estimation of fecal output using chromium dioxide as external marker. However, quantification can be expensive and sometimes not precise due to the methodology used for this purpose. Therefore, the aim of this study was to validate the colorimetric method for chromium quantification and to implement it in the estimation of fecal output in grazing cattle. The temperature, the digestion time and the wavelength for the measurement were evaluated. The method was validated for selectivity, linearity, detection and quantification limits, precision, accuracy, and stability. Results showed that temperature and digestion time are critical to improve sensitivity and quantification limits. The validation demonstrated that the method is suitable for the quantification of Cr2O3 in a wide range of concentrations, being statistically comparable with a reference method, and offering a reliable low cost and easy to implement alternative, to estimate fecal output in bovine digestibility studies.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/48096
10.4025/actascianimsci.v42i1.48096
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| Editor: |
Editora da Universidade Estadual de Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/48096/751375149787
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Animal Sciences; Vol 42 (2020): Publicação contínua; e48096
Acta Scientiarum. Animal Sciences; v. 42 (2020): Publicação contínua; e48096
1807-8672
1806-2636
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| Direitos: |
Copyright (c) 2020 Acta Scientiarum. Animal Sciences
https://creativecommons.org/licenses/by/4.0
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