| Registro completo |
| Provedor de dados: |
Organic Eprints
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| País: |
Germany
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| Título: |
Characterization of arbuscular mycorrhizal communities in roots of vineyard plants
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| Autores: |
Drain, Alice
Bonneau, Laurent
Recorbet, Ghislaine
van Tuinen, Diederik
Wipf, Daniel
Courty, Pierre-Emmanuel
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| Data: |
2019
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| Ano: |
2019
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| Palavras-chave: |
Crop combinations and interactions Biodiversity and ecosystem services Crop health
Quality
Protection Viticulture
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| Resumo: |
We developed a new protocol to study arbuscular mycorrhizal fungal (AMF) communities in Bordeaux vineyards in a standardized way, in order to compare results obtained over years and between locations. To this end, we first used grapevine root samples instead of soil samples to avoid AMF spores or hyphae interacting with cover plants. We next increased the number of grapevine samples to obtain more representative coverage of AMF communities while decreasing variability intra-vineyard, especially for the larger parcels. In addition, we adapted the DNA extraction protocol dedicated to soil samples to grapevine roots, as a way to increase the yield and the purity of samples. These features, coupled to the choice of the LSU-D2 region as the molecular marker for highthroughput sequencing (MiSeq® technology), further allowed us to assess the AMF populations of Bordeaux vineyards.
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| Tipo: |
Book chapter
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| Identificador: |
Drain, Alice; Bonneau, Laurent; Recorbet, Ghislaine; van Tuinen, Diederik; Wipf, Daniel and Courty, Pierre-Emmanuel (2019) Characterization of arbuscular mycorrhizal communities in roots of vineyard plants. In: Methods in Rhizosphere Biology Research. Rhizosphere Biology. Springer Nature Singapore Pte Ltd., Singapore, chapter 3, pp. 27-34.
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| Editor: |
Springer Nature Singapore Pte Ltd.
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| Relação: |
http://orgprints.org/36380/
https://doi.org/10.1007/978-981-13-5767-1_3
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