| Registro completo |
| Provedor de dados: |
Sci. Agrar.Paran. / SAP
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| País: |
Brazil
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| Título: |
Induction of somatic embryogenesis and plant regeneration of sugarcane cv. RB925345 from somatic embryos
Induction of somatic embryogenesis and plant regeneration of sugarcane cv. RB925345 from somatic embryos
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| Autores: |
Almeida, Rodrigo de Oliveira
Silva, Sérgio Delmar dos Anjos e
Dutra, Leonardo Ferreira
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| Data: |
2018-09-05
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| Ano: |
2018
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| Palavras-chave: |
Saccharum spp.
2 4-dichlorophenoxyacetic acid
Plant tissue culture
Somatic embryogenesis
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| Resumo: |
The aim of this study was to establish a protocol for induction of somatic embryogenesis and regeneration of somatic embryos of sugarcane (cultivar RB925345). Best results was obtained using the concentration of 13.5 µM L-1 2,4-dichlorophenoxyacetic acid, 45 days of incubation in induction medium, one subculture and 15 days of incubation in regeneration medium, providing 47.5% of embryogenic calli and 38.9% of plants regenerated. Adjust are necessary in this protocol to increase the rate of embryogenic calli production and regeneration of somatic embryos.
The aim of this study was to establish a protocol for induction of somatic embryogenesis and regeneration of somatic embryos of sugarcane (cultivar RB925345). Best results was obtained using concentration of 13.5 µM of 2,4-dichlorophenoxyacetic acid, 45 days of incubation on induction medium, one subculture and 15 days of incubation on regeneration medium, providing 47.5% of embryogenic calli and 38.9% of plants regenerated. Adjustments are necessary to this protocol to improve the embryogenic calli production rate and regeneration of somatic embryos.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Português
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| Identificador: |
http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14165
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| Editor: |
Scientia Agraria Paranaensis
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| Relação: |
http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14165/13192
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| Formato: |
application/pdf
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| Fonte: |
Scientia Agraria Paranaensis; Vol. 17, No 2 (2018); 165
1983-1471
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| Direitos: |
http://creativecommons.org/licenses/by-nc-sa/4.0
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