| Registro completo |
| Provedor de dados: |
OMA
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| País: |
Belgium
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| Título: |
Sequential determination of a combined gamma/ß and pure ß-emitter by gamma and liquid scintillation counting: application to the transport of metals across fish gills
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| Autores: |
Van Ginneken, L.
Blust, R.
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| Data: |
1995
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| Ano: |
1995
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| Resumo: |
A combined gamma scintillation/liquid scintillation technique for the sequential determination of two radioactive tracers is described. The method was developed using a combined gamma/ß-emitter (<sup>57</sup>Co) and a pure ß-emitter (<sup>45</sup>Ca). First, the <sup>57</sup>Co radioactivity was determined by counting the samples in a gamma scintillation analyzer in the energy region 80-165 keV. Next, the samples were counted in a liquid scintillation analyzer. Only one energy region was used to count both isotopes to maximize the counting efficiencies. From the difference between quenched and unquenched ß-spectra, the counting region was set from 0 to 256 keV. The counting efficiency was related to a quench-indicating parameter (tSIE) for both nuclides by fitting a rectangular hyperbola to the quench data. By subtracting the <sup>57</sup>Co counts from the observed counts in the total window, <sup>45</sup>Ca dpm values were obtained. It is shown that the method presented gives reliable and consistent results. The recoveries of both isotopes are independent of the quench level in a large tSIE range, although five times more radioactivity is required for <sup>45</sup>Ca than for <sup>57</sup>Co to obtain accurate and reproducible results. The method has been used to study mechanisms of metal transport across biological interfaces.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2650
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| Fonte: |
%3Ci%3EAnalytical+Biochemistry+224%3C%2Fi%3E%3A+92-99
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| Direitos: |
info:eu-repo/semantics/restrictedAccess
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