| Registro completo |
| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
Development and characterization of microsatellite markers for Tibouchina hatschbachii (Melastomataceae), an endemic and habitat-restricted shrub from Brazil
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| Autores: |
Maia, Fabiano Rodrigo
Sujii, Patricia Sanae
Goldenberg, Renato
Pereira, Viviane Silva
Zucchi, Maria Imaculada
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| Data: |
2016-12-08
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| Ano: |
2016
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| Palavras-chave: |
Genética Melastomataceae
Tibouchina
Granite
Sandstone
Rock outcrops
Population genetics
Simple sequence repeat (SSR) Marcadores microssatélites
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| Resumo: |
Tibouchina hatschbachii Wurdack (Melastomataceae) is an autogamous shrub restricted to granite (GO) and sandstone (SO) rock outcrops from subtropical Brazil. We designed primers for the amplification of microsatellite regions for T. hatschbachii, and characterized these primers to estimate genetic diversity parameters and contemporary genetic structure patterns. Eight loci were successfully amplified and were characterized using 70 individuals from three natural populations. Polymorphic information content ranged from 0.200 to 0.772 per locus. All loci were polymorphic, with allele numbers ranging from two to eight. The low degree of polymorphism may be explained by the fact that T. hatschbachii has disjunct populations and a recent genetic bottleneck, and also that it is self-pollinated. The observed and expected heterozygosities ranged from 0.115 to 1.000 and from 0.112 to 0.800, respectively. We observed private alleles in all loci. These are important features that enable us to identify population differentiation and help to us understand gene flow patterns for T. hatschbachii in subtropical Brazil. Eight microsatellite loci from other species of Tibouchina amplified positively in T. hatschbachii.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31496
10.4025/actascibiolsci.v38i3.31496
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31496/pdf
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 38, n. 3 (2016); 327-332
Acta Scientiarum. Biological Sciences; v. 38, n. 3 (2016); 327-332
1807-863X
1679-9283
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| Direitos: |
Direitos autorais 2016 Acta Scientiarum. Biological Sciences
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