| Registro completo |
| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
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| Autores: |
Gomes, Lucas Roberto Pereira
Franceschi, Cristina do Rosário Batista
Ribas, Luciana Lopes Fortes
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| Data: |
2015-08-06
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| Ano: |
2015
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| Palavras-chave: |
Biotecnologia
Botânica epiphytic orchid
In vitro propagation
Protocorms
Protocorm-like body 2.03.00.00-0
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| Resumo: |
An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival).
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276
10.4025/actascibiolsci.v37i2.27276
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276/pdf_49
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 37, n. 2 (2015); 143-149
Acta Scientiarum. Biological Sciences; v. 37, n. 2 (2015); 143-149
1807-863X
1679-9283
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