| Registro completo |
| Provedor de dados: |
Biological Sciences
|
| País: |
Brazil
|
| Título: |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2
|
| Autores: |
Oliveira, Luciana Ribeiro Coutinho; Universidade Estadual do Norte Fluminense
Barbosa, João Batista; Universidade Estadual do Norte Fluminense
Martins, Meire Lelis Leal; Universidade Estadual do Norte Fluminense
Martins, Marco Antonio; Universidade Estadual do Norte Fluminense
|
| Data: |
2014-05-08
|
| Ano: |
2014
|
| Palavras-chave: |
5.07.03.00-9 Avicelase
Corn steep liquor
Sugarcane bagasse
Bacillus sp Tecnologia de Alimentos
|
| Resumo: |
Nowadays, the isolation of new bacterial strains that produce enzymes with novel properties is a subject of great relevance to the scientific community. This study, in order to search for producers of new cellulase strains, investigated the avicelase production by thermophilic Bacillus sp. strain SMIA-2. The best avicelase activity was observed in a culture medium containing 0.5% (w v-1) avicel and 0.5% (w v-1) corn steep liquor with initial pH 7.5-8.0 incubated at 50oC. When avicel was replaced in the medium by the treated sugarcane bagasse (0.5%, w v-1) the avicelase activity levels were not affected. Studies on the avicelase characterization revealed that the optimum pH of the enzyme was found to be 8.5 and the enzyme retained more than 80% of its activity after incubation at room temperature for 2h at pH 6.5-8.5. The optimum temperature of this enzyme was 70oC and the enzyme retained 67% of the original activity after 20 min. of heat treatment at 70oC. Avicelase was stimulated by Mn2+ and Co2+, whereas Hg2+ greatly inhibited the enzyme activity
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827
10.4025/actascibiolsci.v36i2.17827
|
| Editor: |
Universidade Estadual De Maringá
|
| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827/pdf_12
|
| Formato: |
application/pdf
|
| Fonte: |
Acta Scientiarum. Biological Sciences; v. 36, n. 2 (2014); 215-222
Acta Scientiarum. Biological Sciences; v. 36, n. 2 (2014); 215-222
1807-863X
1679-9283
|
| Direitos: |
DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).
|
|
