| Registro completo |
| Provedor de dados: |
3
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| País: |
Brazil
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| Título: |
Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
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| Autores: |
Oliveira, Luana Paula de Azevedo de
Oliveira, Tiago Felipe
Contiero, Jonas
Cazetta, Márcia Luciana
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| Data: |
2016-12-08
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| Ano: |
2016
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| Palavras-chave: |
2.12.02.00-1 yeast
Microbial enzymes
Agricultural substrate Microbiologia aplicada
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| Resumo: |
The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489
10.4025/actascibiolsci.v38i3.31489
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489/pdf
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 38, n. 3 (2016); 283-289
Acta Scientiarum. Biological Sciences; v. 38, n. 3 (2016); 283-289
1807-863X
1679-9283
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| Direitos: |
Direitos autorais 2016 Acta Scientiarum. Biological Sciences
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