| Registro completo |
| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation
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| Autores: |
Barcellos, Priscila Gomes
Rodrigues, José Ariévilo Gurgel
Queiroz, Ismael Nilo Lino de
Araújo, Ianna Wivianne Fernandes de
Benevides, Norma Maria Barros
Mourão, Paulo Antônio de Souza
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| Data: |
2018-11-30
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| Ano: |
2018
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| Palavras-chave: |
Ulvales
Complex glycans
Chemical analysis
Thrombosis.
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| Resumo: |
Ulva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physical-chemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, glucose, glucuronic acid and α-/β-types glycosidic linkages as characterized by one-/two-dimensions nuclear magnetic resonance (NMR) experiments. Fractionation of the Ul-SPs by DEAE-cellulose chromatography yielded Ul-SP1 and Ul-SP2 (0.50 and 0.75 M NaCl, respectively) showing sulfation (15.72-18.04%) and total sugars (59.73-60.58%) consistent with the charge density pattern by combination of agarose/polyacrylamide gel eletrophoresis using sequential staining with toluidine blue and stains-all, although with slight differences in their sizes (40 and >100 kDa, respectively). By both activated partial thromboplastin time (APTT) and prothrombin time (PT) tests, anticoagulation of the fractions was virtually detected by APTT (0.39 and 0.43 IU, respectively) against heparin (193 IU). Fractions acted differently on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with 50% efficacies up to 8.3 µg, whereas at high concentrations suggested intrinsic hypercoagulability since heparin abolished both systems at low amounts. Ul-SPs block TG, but predicting thrombosis in increasing doses.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916
10.4025/actascibiolsci.v40i1.34916
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916/pdf
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/34916/14062
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34916
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34916
1807-863X
1679-9283
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| Direitos: |
Direitos autorais 2018 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
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