| Registro completo |
| Provedor de dados: |
Biological Sciences
|
| País: |
Brazil
|
| Título: |
OPTIMIZATION OF THE CULTURE CONDITIONS FOR TANNASE PRODUCTION BY Aspergillus sp. GM4 UNDER SOLID-STATE FERMENTATION
Optimization of culture conditions for tannase production by Aspergillus sp. gm4 in solid state fermentation
|
| Autores: |
Souza, Patrícia Nirlane da Costa
Maia, Natália da Costa
Guimarães, Luís Henrique Souza
Resende, Mário Lúcio Vilela de
Cardoso, Patrícia Gomes
|
| Data: |
2015-03-10
|
| Ano: |
2015
|
| Palavras-chave: |
Surface response methodology
Enzyme
Jamun
Agricultural residues
|
| Resumo: |
The production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF) was investigated using different vegetables leaves such as mango, jamun, coffee and agricultural residues such as coffee husks, rice husks and wheat bran. Among substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. The best incubation time was studied and others variables were optimized using the Central Composite Design. The best conditions for tannase production were: incubation time of 2 days; tannic acid 1.53% (w/w) and potassium nitrate 2.71% (w/w). After the optimization process, the tannase production increased 4.65-fold.Keywords: surface response methodology; enzyme; jamun
Tannase is an industrially important enzyme produced by a large number of microorganisms. This study analyzed the production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF) using different vegetable leaves (mango, jamun and coffee) and agricultural residues (coffee husks, rice husks and wheat bran). Among the substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. A lower incubation time was fixed and supplementation with potassium nitrate and tannic acid were optimized using the Central Composite Design. The best conditions for tannase production were: incubation time of 2 days; tannic acid at 1.53% (w w-1) and potassium nitrate at 2.71% (w w-1). After the optimization process, tannase production increased 4.65-fold, which showed that the statistical experimental design offers a practicable approach to the implementation of optimization of tannase production.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/22731
10.4025/actascibiolsci.v37i1.22731
|
| Editor: |
Universidade Estadual De Maringá
|
| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/22731/pdf_46
|
| Formato: |
application/pdf
|
| Fonte: |
Acta Scientiarum. Biological Sciences; v. 37, n. 1 (2015); 23-30
Acta Scientiarum. Biological Sciences; v. 37, n. 1 (2015); 23-30
1807-863X
1679-9283
|
