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| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR - doi: 10.4025/actascibiolsci.v32i2.4788
Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR - doi: 10.4025/actascibiolsci.v32i2.4788
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| Autores: |
Nascente, Patrícia da Silva; Universidade Federal de pelotas
Coimbra, Helen Silveira; Universidade Federal de Pelotas
Meinerz, Ana Raquel Mano; Universidade Federal de Pelotas
Meireles, Mário Carlos Araújo; Universidade Federal de Pelotas
Mello, João Roberto Braga; Universidade Federal do Rio Grande do Sul
Schuch, Luiz Filipe Damé; Universidade Federal de Pelotas
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| Data: |
2010-05-28
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| Ano: |
2010
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| Palavras-chave: |
2.00.00.00-6
2.08.04.00-8
2.12.00.00-9
2.12.01.03-0 Malassezia pachydermatis
Molecular biology
Otitis externa
Dogs Ciências Biológicas
Biologia Molecular
Microbiologia
Micologia Malassezia pachydermatis
Molecular biology
Otitis externa
Dogs
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| Resumo: |
Several methodologies in molecular biology have been used in the investigation of Malassezia pachydermatis and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiological study of the yeast. The aim of this study was to identify molecular differences in M. pachydermatis samples isolated from dogs with otitis externa. The M. pachydermatis strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A variation was observed in the number and arrangement of the bands among the 49 studied isolates, grouped into nine patterns. Isolate groupings were not found to be related to animal breed, age or sex. It was concluded that M. pachydermatis has differences in its molecular profile, as shown by the molecular technique (RAPD – PCR), which allows isolates to be classified into nine subtypes.
Several methodologies in molecular biology have been used in the investigation of Malassezia pachydermatis and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiological study of the yeast. The aim of this study was to identify molecular differences in M. pachydermatis samples isolated from dogs with otitis externa. The M. pachydermatis strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A variation was observed in the number and arrangement of the bands among the 49 studied isolates, grouped into nine patterns. Isolate groupings were not found to be related to animal breed, age or sex. It was concluded that M. pachydermatis has differences in its molecular profile, as shown by the molecular technique (RAPD – PCR), which allows isolates to be classified into nine subtypes.
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| Tipo: |
Estudo molecular
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| Idioma: |
Português
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4788
10.4025/actascibiolsci.v32i2.4788
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| Editor: |
Universidade Estadual De Maringá
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| Formato: |
application/pdf
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 32, n. 2 (2010); 169-173
Acta Scientiarum. Biological Sciences; v. 32, n. 2 (2010); 169-173
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| Direitos: |
DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).
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