| Registro completo |
| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
Behavior of α-tomatine and tomatidine against several genera of trypanosomatids from insects and plants and Trypanosoma cruzi
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| Autores: |
Evangelista, Adriane Feijó
Kavati, Erica Akemi
Jankevicius, José Vitor
Menolli, Rafael Andrade
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| Data: |
2018-11-30
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| Ano: |
2018
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| Palavras-chave: |
2.13.01.00-0 tomato alkaloids
Trypanocidal activity
Phytomonas
Leptomonas
Crithidia
Herpetomonas. Protozoologia
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| Resumo: |
Glycoalkaloids are important secondary metabolites accumulated by plants as protection against pathogens. One of them, α-tomatine, is found in high concentrations in green tomato fruits, while in the ripe fruits, its aglycone form, tomatidine, does not present a protective effect, and it is usual to find parasites of tomatoes like Phytomonas serpens in these ripe fruits. To investigate the sensitivity of trypanosomatids to the action of α-tomatine, we used logarithmic growth phase culture of 20 trypanosomatids from insects and plants and Trypanosoma cruzi. The lethal dose 50% (LD50) was determined by mixing 107 cells of the different isolates with α-tomatine at concentrations ranging from 10-3 to 10-8 M for 30 min at room temperature. The same tests performed with the tomatidine as a control showed no detectable toxicity against the same trypanosomatid cultures. The tests involved determination of the percentage (%) survival of the protozoan cultures in a Neubauer chamber using optical microscopy. The LD50 values varied from 10-4 to 10-6 M α-tomatine. Slight differences were detected among the LD50 values of the analyzed samples, and none of them showed evidence of resistance to the action of tomatinase, as shown by some pathogenic fungi.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/41853
10.4025/actascibiolsci.v40i1.41853
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/41853/pdf
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/41853/17448
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e41853
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e41853
1807-863X
1679-9283
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| Direitos: |
Direitos autorais 2018 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
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