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Provedor de dados:  Acta Botanica
País:  Brazil
Título:  Cyrtopodium paludicolum germination with two Tulasnella isolates
Autores:  Carvalho,Otieres Cirino de
Paiva Neto,Vespasiano Borges de
Padilha,Daly Roxana Castro
Veloso,Tomás Gomes Reis
Bocayuva,Melissa Faust
Soares,Deanna Carla Oliveira
Kasuya,Maria Catarina Megumi
Data:  2018-03-01
Ano:  2018
Palavras-chave:  Cerrado
ITS-DNAr
Mycorrhiza
Orchidaceae
Symbiosis
Resumo:  ABSTRACT Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. The aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its efficiency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. The experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. The mycobiont CH01 was successfully isolated from Cyrtopodium paludicolum and identified as Tulasnella sp. Treatments with both fungi reached a higher germination percentage than under asymbiotic conditions, indicating no specificity in the relationship between Cyrtopodium paludicolum and the fungi. The results presented have the potential to advance research into the propagation and conservation of C. paludicolum, a native of the Cerrado biome.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100107
Editor:  Sociedade Botânica do Brasil
Relação:  10.1590/0102-33062017abb0181
Formato:  text/html
Fonte:  Acta Botanica Brasilica v.32 n.1 2018
Direitos:  info:eu-repo/semantics/openAccess
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