| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS
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| Autores: |
SOUZA,JOANNE M.M.
BERKOV,STRAHILL
SANTOS,ALBERDAN S.
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| Data: |
2014-09-01
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| Ano: |
2014
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| Palavras-chave: |
Anti-inflammatory lipids
Boerhaavia paniculata
Callus culture
Lipid profile
Medicinal plant
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| Resumo: |
In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m–2 s–1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and glucose (1.5 %; w/v). Lipidomics techniques were applied in hexane fraction showing higher concentrations of the steroids β-sitosterol (3.53 mg/100 g dc–dry cells), and fatty acids, especially 2-hydroxy-tetracosanoic acid (0.34 mg/100 g dc), eicosanoic acid (86.25 mg/100 g dc), stearic acid (420.83 mg/100 g dc), tetradecanoic acid (10.74 mg/100 g dc) and linoleic acid (100.61 mg/100 g dc). The lipid profile of callus versus that found in the roots of wild plant is described in this work.
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301015
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/0001-3765201420130098
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.86 n.3 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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