| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate
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| Autores: |
Almeida,Andréa M.
Bertoncini,Clélia R.A.
Borecký,Jiri
Souza-Pinto,Nadja C.
Vercesi,Aníbal E.
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| Data: |
2006-09-01
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| Ano: |
2006
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| Palavras-chave: |
Iron
Lipid peroxidation
Mitochondria
MtDNA
Oxidative stress
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| Resumo: |
Iron imbalance/accumulation has been implicated in oxidative injury associated with many degenerative diseases such as hereditary hemochromatosis, beta-thalassemia, and Friedreich's ataxia. Mitochondria are particularly sensitive to iron-induced oxidative stress - high loads of iron cause extensive lipid peroxidation and membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrial DNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weight complex. Intense DNA fragmentation was induced after the incubation of mitochondria with the iron complex. The detection of 3' phosphoglycolate ends at the mtDNA strand breaks by a 32P-postlabeling assay, suggested the involvement of hydroxyl radical in the DNA fragmentation induced by Fe2+-citrate. Increased levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine also suggested that Fe2+-citrate-induced oxidative stress causes mitochondrial DNA damage. In conclusion, our results show that iron-mediated lipid peroxidation was associated with intense mtDNA damage derived from the direct attack of reactive oxygen species.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300010
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/S0001-37652006000300010
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.78 n.3 2006
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| Direitos: |
info:eu-repo/semantics/openAccess
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