| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Multidrug resistance in tumour cells: characterisation of the multidrug resistant cell line K562-Lucena 1
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| Autores: |
RUMJANEK,VIVIAN M.
TRINDADE,GILMA S.
WAGNER-SOUZA,KAREN
MELETTI-DE-OLIVEIRA,MICHELE C.
MARQUES-SANTOS,LUIS F.
MAIA,RAQUEL C.
CAPELLA,MÁRCIA A. M.
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| Data: |
2001-03-01
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| Ano: |
2001
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| Palavras-chave: |
Multidrug resistance
Leukaemia
P-glycoprotein
Chemosensitisers
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| Resumo: |
Multidrug resistance to chemotherapy is a major obstacle in the treatment of cancer patients. The best characterised mechanism responsible for multidrug resistance involves the expression of the MDR-1 gene product, P-glycoprotein. However, the resistance process is multifactorial. Studies of multidrug resistance mechanisms have relied on the analysis of cancer cell lines that have been selected and present cross-reactivity to a broad range of anticancer agents. This work characterises a multidrug resistant cell line, originally selected for resistance to the Vinca alkaloid vincristine and derived from the human erythroleukaemia cell K562. This cell line, named Lucena 1, overexpresses P-glycoprotein and have its resistance reversed by the chemosensitisers verapamil, trifluoperazine and cyclosporins A, D and G. Furthermore, we demonstrated that methylene blue was capable of partially reversing the resistance in this cell line. On the contrary, the use of 5-fluorouracil increased the resistance of Lucena 1. In addition to chemotherapics, Lucena 1 cells were resistant to ultraviolet A radiation and hydrogen peroxide and failed to mobilise intracellular calcium when thapsigargin was used. Changes in the cytoskeleton of this cell line were also observed.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000100007
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/S0001-37652001000100007
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.73 n.1 2001
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| Direitos: |
info:eu-repo/semantics/openAccess
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