| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
In vitro antioxidant, DNA-damaged protection and antiproliferative activities of ethyl acetate and n-butanol extracts of Centaurea sphaerocephalaL.
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| Autores: |
LAHNECHE,AMINA M.
BOUCHEHAM,RATIBA
OZEN,TEVFIK
ALTUN,MUHAMMED
BOUBEKRI,NASSIMA
DEMIRTAS,IBRAHIM
BICHA,SABRINA
BENTAMENE,ALI
BENAYACHE,FADILA
BENAYACHE,SAMIR
ZAMA,DJAMILA
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| Data: |
2019-01-01
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| Ano: |
2019
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| Palavras-chave: |
Centaurea sphaerocephala
Phenolics
Antioxidant
Lipid peroxidation
Antiproliferative
DNA-damaged inhibition
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| Resumo: |
Abstract: This study aimed to evaluate the in vitro antiproliferative and inhibition of oxidative DNA-damage activities of n-butanol (n-BuOH) extract of Centaurea sphaerocephala. The in vitro antioxidant activity of the ethyl acetate (EtOAc) and the n-BuOH extracts of this plant were also assayed. To investigate the antioxidant potential, extracts were tested for their capacity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH·) and to inhibit lipid peroxidation using the TBARs method. The contents of total phenolics and flavonoids were measured. Additionally, antiproliferative activity and DNA-damage inhibition of the n-BuOH extract was determined using XCELLigence RTCA instrument and photolyzing 46966 plasmid, respectively. The results exhibited that the scavenging abilities of the EtOAc extract were better than the n-BuOH extract with an IC50= 11.59 µg/mL and 16.67 µg/mL for both extracts, respectively. The phenolic and flavonoid contents were found higher in the n-BuOH and EtOAc extracts. Furthermore, our results showed that n-BuOH extract exhibited a remarkable inhibition of lipid peroxidation with an IC50 of 340.94±7.49 μg/mL and had an antiproliferative effect against Hela cells. Extracts of C. sphaerocephala showed antioxidant activity on scavenging DPPH·. In addition, the n-BuOH extract inhibited the lipid peroxidation and exhibited an antiproliferative effect against HeLa cells line (human cervix carcinoma).
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500619
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/0001-3765201920180462
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.91 n.3 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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