Registro completo |
Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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País: |
Brazil
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Título: |
ELISA indireto para detecção de IgG antivírus da doença de Newcastle em soro de codorna
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Autores: |
Oliveira,D.D.
Folgueras-Flatschart,A.V.
Flatschart,R.B.
Resende,J.S.
Abreu,J.T.
Martins,N.R.S.
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Data: |
2007-10-01
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Ano: |
2007
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Palavras-chave: |
Codorna japonesa
ELISA
IgG
Doença de Newcastle
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Resumo: |
An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100%). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation.
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Tipo: |
Info:eu-repo/semantics/other
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Idioma: |
Português
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500040
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Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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Relação: |
10.1590/S0102-09352007000500040
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Formato: |
text/html
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Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.59 n.5 2007
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Direitos: |
info:eu-repo/semantics/openAccess
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