| Registro completo |
| Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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| País: |
Brazil
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| Título: |
Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
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| Autores: |
Domingues,S.F.S.
Ferreira,H.S.
Muniz,J.A.P.C.
Lima,A.K.F.
Ohashi,O.M.
Figueiredo,J.R.
Silva,L.D.M.
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| Data: |
2003-06-01
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| Ano: |
2003
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| Palavras-chave: |
Monkey
Preantral follicle
Isolation
Ovary
Cebus apella
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| Resumo: |
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009
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| Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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| Relação: |
10.1590/S0102-09352003000300009
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| Formato: |
text/html
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| Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.55 n.3 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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