Registro completo |
Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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País: |
Brazil
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Título: |
Use of an ELISA system for detection of equine herpesvirus 1 (EHV-1) antibodies in non-symptomatic pregnant mares and neonatal foals
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Autores: |
Carvalho,R.
Passos,L.M.F.
Gouvea,A.M.G.
Resende,M.
Martins,A.S.
Franco,G.C.
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Data: |
2000-06-01
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Ano: |
2000
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Palavras-chave: |
Horse
Herpesvirus
ELISA
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Resumo: |
An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000300003
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Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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Relação: |
10.1590/S0102-09352000000300003
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Formato: |
text/html
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Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.52 n.3 2000
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Direitos: |
info:eu-repo/semantics/openAccess
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