| Registro completo |
| Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
|
| País: |
Brazil
|
| Título: |
Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate
|
| Autores: |
Fonseca,J.F.
Batista,R.I.T.P.
Souza-Fabjan,J.M.G.
Oliveira,M.E.F.
Brandão,F.Z.
Viana,J.H.M.
|
| Data: |
2018-10-01
|
| Ano: |
2018
|
| Palavras-chave: |
Embryo survival
Embryo transfer
Goat
Slow freezing
|
| Resumo: |
ABSTRACT The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were maintained in holding medium (37ºC). Fresh and frozen-thawed embryos were transferred at day 7 post-estrus to 30 recipients. Kidding and kid born rates were similar (P> 0.05), respectively, for recipients receiving fresh (66.7% or 10/15; 55.2% or 16/29) or frozen-thawed (60% or 9/15; 51.7% or 15/29) embryos. The cryopreservation of goat embryos using slow-freezing protocol and 1.5MEG resulted in similar efficiency rates of fresh embryos.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501489
|
| Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
|
| Relação: |
10.1590/1678-4162-10196
|
| Formato: |
text/html
|
| Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.70 n.5 2018
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
