| Registro completo |
| Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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| País: |
Brazil
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| Título: |
Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed
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| Autores: |
Sprícigo,J.F.W.
Leme,L.O.
Guimarães,A.L.
Oliveira Neto,J.C.
Silva,P.C.P.
Moreira,N.H.
Pivato,I.
Silva,B.D.M.
Ramos,A.F.
Dode,M.A.N.
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| Data: |
2019-06-01
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| Ano: |
2019
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| Palavras-chave: |
Pig
Embryo
Cryopreservation
Lipids
Apoptosis
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| Resumo: |
ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming, blastocysts were in vitro cultured to assess re-expansion and hatching at 24 and 48 hours. Finally, at 48 hours, hatched blastocysts were submitted to RT-qPCR searching for BCL2A1, BAK, BAX and CASP3 genes. For MALDI-TOF, the ion intensity was expressed in arbitrary units. Blastocyst development was compared by Qui-square (P< 0.05). Among the most representative PL was the phosphatidylcholine [PC (32:0) + H]+; [PC (34:1) + H]+ and [PC (36:4) + H]+. Beyond the PL, MALDI revealed some triglycerides (TG), including PPL (50:2) + Na+, PPO (50:1) + Na+, PLO (52:3) + Na+ and POO (52:2) + Na. Re-expansion did not differ (P> 0.05) between fresh or vitrified blastocysts at 24 (33.3%; 32.7%) or 48 hours (2.4%; 13.5%). Hatching rates were higher (P< 0.05) for fresh compared to vitrified at 24 (66.7%; 15.4%) and 48 hours (97.6%; 36.0%). BAX was overexpressed (P< 0.05) after vitrification. In conclusion, Piau blastocysts can be cryopreserved by Cryotop. This study also demonstrated that the apoptotic pathway may be responsible for the low efficiency of porcine embryo cryopreservation.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300837
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| Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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| Relação: |
10.1590/1678-4162-10249
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| Formato: |
text/html
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| Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.3 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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