| Registro completo |
| Provedor de dados: |
Biocell
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| País: |
Argentina
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| Título: |
Structure and secretory activity of cultured chondrocytes from patients with osteoarthritis
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| Autores: |
Montrull,Hilda L.
Brizuela,Nilda Y.
Demurtas,Silvia L.
Spitale,Luis
Meirovich,Carlos I.
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| Data: |
2005-08-01
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| Ano: |
2005
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| Palavras-chave: |
Osteoarthritis
Chondrocytes
Collagenase
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| Resumo: |
Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco's modified Eagle medium at 37°C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO-2 and NO-3 were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA. The concentration of NO-x was 47.3 ± 4.1 µM in the OA cartilague and 10.7 ± 1.8 µM in the controls. The average MMP-1 activity was 3,650 ± 387 ng/ml in the OA cartilage and 2,150 ± 190 ng/ml in the control samples. These increased values of MMP-1 and NO-x observed in the OA cartilage suggest a higher catabolic activity. A morphological analysis of OA chondral tissue using light microscopy shows that the surface of the tissue is characterized by the presence of aggregated chondrocytes or &"clones&" but in the deeper areas isolated cells are found. These results could be a significant contribution towards the identification of biological markers indicating the presence of OA activity.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200002
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| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
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| Formato: |
text/html
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| Fonte: |
Biocell v.29 n.2 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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