| Registro completo |
| Provedor de dados: |
Biocell
|
| País: |
Argentina
|
| Título: |
Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli
|
| Autores: |
Rüttler,M.E.
Yanzón,C.S.
Cuitiño,M.J.
Renna,N.F.
Pizarro,M.A.
Ortiz,A.M.
|
| Data: |
2006-08-01
|
| Ano: |
2006
|
| Palavras-chave: |
Diarrhea
Escherichia coli
Cell culture
PCR (Polymerase Chain Reaction)
|
| Resumo: |
Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator ( aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 ( astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a multiplex PCR assay which checks aggR and astA genes was designed. Eigthy- eight E.coli strains, isolated from children with acute diarrhea in Mendoza , Argentina , were characterized by the reference method (HEp-2 assay), and by aggRastA PCR. A strong correlation between the presence of the specific marker aggR and the reference test was found. The astA gene had a similar distribution between aggregative and localized strains, indicating that this gene could not be considered as a marker of EAEC. We conclude that aggR may be used to identify EAEC, using the PCR method as a screening test.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200006
|
| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
|
| Formato: |
text/html
|
| Fonte: |
Biocell v.30 n.2 2006
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
|
