| Registro completo |
| Provedor de dados: |
Biocell
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| País: |
Argentina
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| Título: |
High passage numbers induce resistance to apoptosis in C2C12 muscle cells
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| Autores: |
Pronsato,Lucia
La Colla,Anabela
Ronda,Ana Carolina
Milanesi,Lorena
Boland,Ricardo
Vasconsuelo,Andrea
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| Data: |
2013-04-01
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| Ano: |
2013
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| Palavras-chave: |
Over-subculturing changes
Aged muscle cultures
Mitochondrial DNA
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| Resumo: |
Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100001
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| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
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| Formato: |
text/html
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| Fonte: |
Biocell v.37 n.1 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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