| Registro completo |
| Provedor de dados: |
Biocell
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| País: |
Argentina
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| Título: |
Influence of 2-methoxyestradiol on MCF-7 cells: An improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels
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| Autores: |
Joubert,Annie
Marais,Sumari
Maritz,Christine
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| Data: |
2009-04-01
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| Ano: |
2009
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| Palavras-chave: |
Breast carcinoma
Mitosis
Apoptosis
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| Resumo: |
Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 μM 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these proteins may be time- and cell type dependent and thus not evident during the mitotic block observed. Investigation of the regulation of the B-cell lymphoma 2 family will allow researchers to consider signaling pathways for diseases where apoptosis can potentially be controlled.
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| Tipo: |
Info:eu-repo/semantics/report
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100007
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| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
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| Formato: |
text/html
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| Fonte: |
Biocell v.33 n.1 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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