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Provedor de dados: |
Biol. Res.
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País: |
Chile
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Título: |
Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
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Autores: |
Guo,Jian-Ru
Chen,Qian-Qian
Lam,Christopher Wai-Kei
Zhang,Wei
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Data: |
2015-01-01
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Ano: |
2015
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Palavras-chave: |
Karanjin
Cell cycle
Apoptosis
Cancer therapy
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Resumo: |
BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULTS: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late apoptosis cells. CONCLUSION: Karanjin can induce cancer cell death through cell cycle arrest and enhance apoptosis. This compound may be effective clinically for cancer pharmacotherapy.
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Tipo: |
Journal article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040
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Editor: |
Sociedad de Biología de Chile
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Formato: |
text/html
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Fonte: |
Biological Research v.48 2015
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