| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Molecular analyses of protists in long-term observation programmes—current status and future perspectives
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| Autores: |
Stern, Rowena
Kraberg, Alexandra
Bresnan, Eileen
Kooistra, Wiebe H. C. F.
Lovejoy, Connie
Montresor, Marina
Moran, Xose Anxelu G.
Not, Fabrice
Salas, Rafael
Siano, Raffaele
Vaulot, Daniel
Amaral-zettler, Linda
Zingone, Adriana
Metfies, Katja
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| Data: |
2018-09
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| Ano: |
2018
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| Palavras-chave: |
Protists
Long-term Ecological Research Station
Molecular
Time-series
Questionnaire
Literature survey
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| Resumo: |
Protists (microbial eukaryotes) are diverse, major components of marine ecosystems, and are fundamental to ecosystem services. In the last 10 years, molecular studies have highlighted substantial novel diversity in marine systems including sequences with no taxonomic context. At the same time, many known protists remain without a DNA identity. Since the majority of pelagic protists are too small to identify by light microscopy, most are neither comprehensively or regularly taken into account, particularly in Long-term Ecological Research Sites. This potentially undermines the quality of research and the accuracy of predictions about biological species shifts in a changing environment. The ICES Working Group for Phytoplankton and Microbial Ecology conducted a questionnaire survey in 2013–2014 on methods and identification of protists using molecular methods plus a literature review of protist molecular diversity studies. The results revealed an increased use of high-throughput sequencing methods and a recognition that sequence data enhance the overall datasets on protist species composition. However, we found only a few long-term molecular studies and noticed a lack of integration between microscopic and molecular methods. Here, we discuss and put forward recommendations to improve and make molecular methods more accessible to Long-term Ecological Research Site investigators.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00455/56708/58470.pdf
https://archimer.ifremer.fr/doc/00455/56708/58471.pdf
https://archimer.ifremer.fr/doc/00455/56708/58472.xlsx
https://archimer.ifremer.fr/doc/00455/56708/58473.xlsx
DOI:10.1093/plankt/fby035
https://archimer.ifremer.fr/doc/00455/56708/
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| Editor: |
Oxford Univ Press
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Plankton Research (0142-7873) (Oxford Univ Press), 2018-09 , Vol. 40 , N. 5 , P. 519-536
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| Direitos: |
The Author(s) 2018. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
info:eu-repo/semantics/openAccess
restricted use
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