| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica
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| Autores: |
Lechable, Marion
Jan, Anne-cecile
Duchene, Axel
Uveira, Julie
Weissbourd, Brandon
Gissat, Loann
Collet, Sophie
Gilletta, Laurent
Chevalier, Sandra
Leclère, Lucas
Peron, Sophie
Barreau, Carine
Lasbleiz, Régis
Houliston, Evelyn
Momose, Tsuyoshi
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| Data: |
2020-11
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| Ano: |
2020
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| Palavras-chave: |
Animal culture
Cnidarian
Developmental biology
Genetics
Jellyfish
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| Resumo: |
The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different feeding regimes using Artemia larvae as food and demonstrate that the stage-dependent feeding control is the key for rapid and reliable medusa and polyp rearing. Metamorphosis of the planula larvae into a polyp colony can be induced efficiently using a new synthetic peptide. The optimized procedures detailed here make it practical to generate genetically modified Clytia strains and to maintain their whole life cycle in the laboratory.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00656/76820/77996.pdf
https://archimer.ifremer.fr/doc/00656/76820/77997.pdf
DOI:10.1242/bio.051268
https://archimer.ifremer.fr/doc/00656/76820/
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| Editor: |
The Company of Biologists
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| Formato: |
application/pdf
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| Fonte: |
Biology Open (2046-6390) (The Company of Biologists), 2020-11 , Vol. 9 , N. 11 , P. bio051268 (13p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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