| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei
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| Autores: |
Labreuche, Yannick
Veloso, Artur
De La Vega, Enrique
Gross, Paul S.
Chapman, Robert W.
Browdy, Craig L.
Warr, Gregory W.
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| Data: |
2010-11
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| Ano: |
2010
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| Palavras-chave: |
Shrimp
Litopenaeus vannamei
RNAi
DsRNA
Argonaute
Sid-1
Antiviral immunity
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| Resumo: |
Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both RNAi-related and innate antiviral responses. They also suggest that these two mechanisms of antiviral response may activate the same pathway, requiring Lv-Sid 1 and Lv-Ago 2 induction. (C) 2010 Elsevier Ltd. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00015/12652/9587.pdf
DOI:10.1016/j.dci.2010.06.017
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| Editor: |
Elsevier Sci Ltd
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| Relação: |
http://archimer.ifremer.fr/doc/00015/12652/
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| Formato: |
application/pdf
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| Fonte: |
Developmental And Comparative Immunology (0145-305X) (Elsevier Sci Ltd), 2010-11 , Vol. 34 , N. 11 , P. 1209-1218
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| Direitos: |
2010 Elsevier Ltd All rights reserved.
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