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Provedor de dados:  ArchiMer
País:  France
Título:  First description of French V. tubiashii strains pathogenic to mollusk: I. Characterization of isolates and detection during mortality events
Autores:  Travers, Marie-agnes
Mersni Achour, Rachida
Haffner, Philippe
Tourbiez, Delphine
Cassone, Anne-laure
Morga, Benjamin
Doghri, Ibtissem
Garcia, Celine
Renault, Tristan
Fruitier-arnaudin, Ingrid
Saulnier, Denis
Data:  2014-11
Ano:  2014
Palavras-chave:  Crassostrea gigas Pathogenicity Extracellular products Polyphasic approach
Real time PCR
Resumo:  Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was demonstrated (41% of mortalities). Moreover, a QPCR diagnostic tool targeting the gyrB gene was developed to investigate the epidemiological significance of V. tubiashii in French oyster mortality outbreaks recorded by the national surveillance network. Of the 21 batches originating from hatcheries, only two were positive, whereas V. tubiashii DNA could not be detected in any of the batches of moribund animals collected in field/outdoor facilities. These results demonstrate the existence of a group of virulent V. tubiashii in France that episodically infect C. gigas.
Tipo:  Text
Idioma:  Inglês

Editor:  Academic Press Inc Elsevier Science
Formato:  application/pdf
Fonte:  Journal Of Invertebrate Pathology (0022-2011) (Academic Press Inc Elsevier Science), 2014-11 , Vol. 123 , P. 38-48
Direitos:  2014 Elsevier Inc. All rights reserved.


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