| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Building of an immunosensor: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?
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| Autores: |
Briand, Elisabeth
Salmain, Michèle
Henry, Jean-marie
Perrot, Hubert
Compere, Chantal
Pradier, Claire-marie
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| Data: |
2006-09
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| Ano: |
2006
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| Palavras-chave: |
QCM
IRRAS
Thiol attachment
Immunosensor
Mixed SAM' s
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| Resumo: |
Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2006/publication-1988.pdf
DOI:10.1016/j.bios.2006.05.018
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/1988/
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| Formato: |
application/pdf
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| Fonte: |
Biosensors and Bioelectronics (0956-5663) (Elsevier), 2006-09 , Vol. 22 , N. 3 , P. 440-448
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| Direitos: |
2006 Elsevier B.V. All rights reserved
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