| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling
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| Autores: |
Vaulot, Daniel
Lepere, Cecile
Toulza, Eve
De La Iglesia, Rodrigo
Poulain, Julie
Gaboyer, Frederic
Moreau, Herve
Vandepoele, Klaas
Ulloa, Osvaldo
Gavory, Frederick
Piganeau, Gwenael
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| Data: |
2012-06
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| Ano: |
2012
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| Resumo: |
Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as Bathycoccus, Micromonas, and Ostreococcus are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of Bathycoccus strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant Bathycoccus genome sequences. WGA did not amplify all regions uniformly, resulting in unequal coverage along a given chromosome and between chromosomes. The identity at the DNA level between the metagenomes and the cultured genome was very high (96.3% identical bases for the three larger chromosomes over a 360 kbp alignment). At least two to three different genotypes seemed to be present in each natural sample based on read mapping to Bathycoccus RCC1105 genome.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00129/23987/21945.pdf
DOI:10.1371/journal.pone.0039648
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| Editor: |
Public Library Science
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| Relação: |
http://archimer.ifremer.fr/doc/00129/23987/
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| Formato: |
application/pdf
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| Fonte: |
Plos One (1932-6203) (Public Library Science), 2012-06 , Vol. 7 , N. 6 , P. -
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| Direitos: |
2012 Vaulot et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This research was supported by the following programs: BIOSOPE cruise (Funded by the Centre National de la
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