| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria
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| Autores: |
Ortega Morales, Benjamín Otto
Santiago Garcia, J.l
Chan Bacab, M.j.
Moppert, Xavier
Miranda Tello, E
Fardeau, M.l.
Carrero, J.c.
Bartolo Perez, P.
Valadez Gonzalez, A
Guezennec, Jean
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| Data: |
2007
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| Ano: |
2007
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| Palavras-chave: |
Tropical intertidal biofilm bacteria
Surfactants
Microbacterium sp
Exopolymers
Bacillus sp
Anionic
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| Resumo: |
Aims: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. EPS MC3B-10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. The chemical composition of polymer MC6B-22 suggests its potential biomedical application in tissue regeneration. Significance and Impact of the Study: This is the first report of a Microbacterium species producing EPS with surfactant properties, which expands our knowledge of the micro-organisms capable of producing these biomolecules. Furthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting EPS-producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2007/publication-2530.pdf
DOI:10.1111/j.1365-2672.2006.03085.x
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| Editor: |
Blackwell science
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2530/
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| Formato: |
application/pdf
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| Fonte: |
Journal of Applied Microbiology (1364-5072) (Blackwell science), 2007 , Vol. 102 , N. 1 , P. 254-264
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| Direitos: |
2006 Blackwell
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