| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit
|
| Autores: |
Garcia-martinez, Gonzalo
Alonso Bustabad, Enrique
Perrot, Hubert
Gabrielli, Claude
Bucur, Bogdan
Lazerges, Mathieu
Rose, Daniel
Rodriguez-pardo, Loreto
Farina, Jose
Compere, Chantal
Arnau Vives, Antonio
|
| Data: |
2011-08
|
| Ano: |
2011
|
| Palavras-chave: |
DNA sequence detection
Quartz crystal microbalance
Electronic oscillator
Sensitivity
Frequency noise
Resolution
|
| Resumo: |
This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
http://archimer.ifremer.fr/doc/00044/15487/12886.pdf
DOI:10.3390/s110807656
|
| Editor: |
Mdpi Ag
|
| Relação: |
http://archimer.ifremer.fr/doc/00044/15487/
|
| Formato: |
application/pdf
|
| Fonte: |
Sensors (1424-8220) (Mdpi Ag), 2011-08 , Vol. 11 , N. 8 , P. 7656-7664
|
| Direitos: |
2011 by the authors; licensee MDPI, Basel, Switzerland.
|
|
