| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Improved procedures for extraction of water-extractable carbohydrates from particulate organic matter
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| Autores: |
Burdloff, D
Etcheber, H
Buscail, R
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| Data: |
2001-07
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| Ano: |
2001
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| Palavras-chave: |
Facteurs d’extraction
Matière organique particulaire
Sucres solubles
Extraction factors
Particulate organic matter
Water-soluble carbohydrates
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| Resumo: |
The present work presents improvements to the existing methods for extraction of carbohydrates with water. In order to test the effects of extraction factors on the yield of carbohydrates, extractions of water-soluble carbohydrates were performed with two contrasting samples (suspended particulate organic matter from the high turbidity zone of the Gironde estuary and sedimentary organic matter from the Arcachon lagoon) under different experimental conditions. Results show that: (i) boiling water applied for 30 min yielded the best result for water-soluble carbohydrates in both samples; (ii) sonic disruption had a strong influence on the recovery rate of water-soluble carbohydrates - a steady yield was reached after a 3-min treatment; (iii) almost all the water-extractable carbohydrates are removed after the third extraction. Consequently, a general method is proposed to improve the extraction of water-extractable carbohydrates from particulate matter.
Sur la base de deux échantillons organiques de nature opposée (matières en suspension de la zone de maximum de turbidité de l'estuaire de la Gironde et sédiments du bassin d'Arcachon), on a pu évaluer les effets de différents facteurs d'extraction sur la méthode d'extraction des sucres par l'eau. Ainsi, le rendement d'extraction des sucres solubles devient optimum (i) lorsque l'extraction est conduite à 100 °C et dans une période minimale de 30 min ; (ii) lors de l'application d'un traitement aux ultrasons de 3 min ; (iii) après trois extractions successives. Une optimisation de l'extraction des sucres solubles est alors proposée en vue de l'harmonisation des méthodes d'extraction.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00322/43347/42879.pdf
DOI:10.1016/S0399-1784(01)01151-3
http://archimer.ifremer.fr/doc/00322/43347/
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| Editor: |
Gauthier-villars/editions Elsevier
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| Formato: |
application/pdf
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| Fonte: |
Oceanologica Acta (0399-1784) (Gauthier-villars/editions Elsevier), 2001-07 , Vol. 24 , N. 4 , P. 343-347
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| Direitos: |
2001 Ifremer/CNRS/IRD/Éditions scientifiques et médicales Elsevier SAS
info:eu-repo/semantics/openAccess
restricted use
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