| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass
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| Autores: |
Masroor, Waliullah
Farcy, Emilie
Blondeau-bidet, Eva
Venn, Alexander
Tambutté, Eric
Lorin-nebel, Catherine
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| Data: |
2019-10
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| Ano: |
2019
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| Palavras-chave: |
Dicentrarchus labrax
Ion transporter
Gill
MRNA expression
Thermal acclimation
Salinity
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| Resumo: |
The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 °C (temperate) or 24 °C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase α1a (nka α1a) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl−-cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl--1 cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00514/62600/66958.pdf
DOI:10.1016/j.jtherbio.2019.102422
https://archimer.ifremer.fr/doc/00514/62600/
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| Editor: |
Elsevier BV
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Thermal Biology (0306-4565) (Elsevier BV), 2019-10 , Vol. 85 , P. 102422 (9p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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