| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia : Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCO genes
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| Autores: |
Duperron, Sebastien
Fiala Medioni, Aline
Caprais, Jean-claude
Olu, Karine
Sibuet, Myriam
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| Data: |
2007
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| Ano: |
2007
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| Palavras-chave: |
Eastern Mediterranean
Cold seeps
Lucinoma
Lucinidae
Sulphide oxidizing bacteria
Symbiosis
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| Resumo: |
Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences encoding the APS reductase alpha subunit and ribulose-1,5-bisphosphate carboxylase oxygenase support the hypothesis that the dominant symbiont can perform sulphide oxidation and autotrophy. Transmission electron micrographs of gills confirmed the dominance of sulphide-oxidizing bacteria, which display typical vacuoles, and delta C-13 values measured in gill and foot tissue further support the hypothesis for a chemoautotrophic-sourced host carbon nutrition.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2007/publication-2176.pdf
DOI:10.1111/j.1574-6941.2006.00194.x
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| Editor: |
Blackwell science
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2176/
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| Formato: |
application/pdf
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| Fonte: |
FEMS Microbiology Ecology (0168-6496) (Blackwell science), 2007 , Vol. 59 , N. 1 , P. 64-70
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| Direitos: |
2007 Blackwell Publishing, Inc.
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