| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Novel inhibition of archaeal family-D DNA polymerase by uracil
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| Autores: |
Richardson, Tomas T.
Gilroy, Louise
Ishino, Yoshizumi
Connolly, Bernard A.
Henneke, Ghislaine
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| Data: |
2013-04
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| Ano: |
2013
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| Resumo: |
Archaeal family-D DNA polymerase is inhibited by the presence of uracil in DNA template strands. When the enzyme encounters uracil, following three parameters change: DNA binding increases roughly 2-fold, the rate of polymerization slows by a factor of similar to 5 and 3'-5' proof-reading exonuclease activity is stimulated by a factor of similar to 2. Together these changes result in a significant decrease in polymerization activity and a reduction in net DNA synthesis. Pol D appears to interact with template strand uracil irrespective of its distance ahead of the replication fork. Polymerization does not stop at a defined location relative to uracil, rather a general decrease in DNA synthesis is observed. 'Trans' inhibition, the slowing of Pol D by uracil on a DNA strand not being replicated is also observed. It is proposed that Pol D is able to interact with uracil by looping out the single-stranded template, allowing simultaneous contact of both the base and the primer-template junction to give a polymerase-DNA complex with diminished extension ability.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00138/24975/23070.pdf
DOI:10.1093/nar/gkt083
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| Editor: |
Oxford Univ Press
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| Relação: |
http://archimer.ifremer.fr/doc/00138/24975/
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| Formato: |
application/pdf
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| Fonte: |
Nucleic Acids Research (0305-1048) (Oxford Univ Press), 2013-04 , Vol. 41 , N. 7 , P. 4207-4218
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| Direitos: |
The Author(s) 2013. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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