| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi
|
| Autores: |
Castillo-lizardo, Melissa
Henneke, Ghislaine
Viguera, Enrique
|
| Data: |
2014-08
|
| Ano: |
2014
|
| Palavras-chave: |
Slippage
Primer-template misalignment
DNA polymerases
Strand displacement activity
Archaea
|
| Resumo: |
Replication slippage or slipped-strand mispairing involves the misalignment of DNA strands during the replication of repeated DNA sequences, and can lead to genetic rearrangements such as microsatellite instability. Here, we show that PolB and PolD replicative DNA polymerases from the archaeal model Pyrococcus abyssi (Pab) slip in vitro during replication of a single-stranded DNA template carrying a hairpin structure and short direct repeats. We find that this occurs in both their wild-type (exo+) and exonuclease deficient (exo-) forms. The slippage behavior of PabPolB and PabPolD, probably due to limited strand displacement activity, resembles that observed for the high fidelity P furiosus (Pfu) DNA polymerase. The presence of PabPCNA inhibited PabPolB and PabPolD slippage. We propose a model whereby PabPCNA stimulates strand displacement activity and polymerase progression through the hairpin, thus permitting the error-free replication of repetitive sequences.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
http://archimer.ifremer.fr/doc/00212/32375/30835.pdf
DOI:10.3389/fmicb.2014.00403
http://archimer.ifremer.fr/doc/00212/32375/
|
| Editor: |
Frontiers Research Foundation
|
| Formato: |
application/pdf
|
| Fonte: |
Frontiers In Microbiology (1664-302X) (Frontiers Research Foundation), 2014-08 , Vol. 5 , N. 403 , P. 1-10
|
| Direitos: |
2014 Frontiers Media S.A. All Rights Reserved
info:eu-repo/semantics/openAccess
restricted use
|
