| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
State of art and best practices for fatty acid analysis in aquatic sciences
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| Autores: |
Couturier, Lydie
Michel, Loic
Amaro, Teresa
Budge, Suzanne M
Da Costa, Elisabete
De Troch, Marleen
Di Dato, Valeria
Fink, Patrick
Giraldo, Carolina
Le Grand, Fabienne
Loaiza, Iván
Mathieu-resuge, Margaux
Nichols, Peter D
Parrish, Christopher C
Sardenne, Fany
Vagner, Marie
Pernet, Fabrice
Soudant, Philippe
Browman, Howard
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| Data: |
2020-12
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| Ano: |
2020
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| Palavras-chave: |
Aquatic nutrition
Biomarkers
Fatty acids
Marine and freshwater lipids
Methods
Recommended practices
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| Resumo: |
Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00651/76279/77252.pdf
DOI:10.1093/icesjms/fsaa121
https://archimer.ifremer.fr/doc/00651/76279/
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| Editor: |
Oxford University Press (OUP)
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| Formato: |
application/pdf
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| Fonte: |
Ices Journal Of Marine Science (1054-3139) (Oxford University Press (OUP)), 2020-12 , Vol. 77 , N. 7-8 , P. 2375-2395
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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