| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Taura syndrome of penaeid shrimp: cloning of viral genome fragments and development of specific gene probes
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| Autores: |
Mari, Jocelyne
Bonami, Jean-robert
Lightner, Donald V
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| Data: |
1998-05
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| Ano: |
1998
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| Palavras-chave: |
TSV
Penaeid shrimp
Picornavirus
Cloning
Dot blot hybridization
In situ hybridization
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| Resumo: |
The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No positive hybridization was obtained with other shrimp viruses tested [Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and Hepatopancreatic Parvovirus (HPV)]. The specificity of the 2 probes was con firmed by in situ hybridization on histological sections of TS diseased shrimps.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00447/55899/57914.pdf
DOI:10.3354/dao033011
http://archimer.ifremer.fr/doc/00447/55899/
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| Editor: |
Inter-research
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| Formato: |
application/pdf
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| Fonte: |
Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1998-05 , Vol. 33 , N. 1 , P. 11-17
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| Direitos: |
Inter-Research 1998
info:eu-repo/semantics/openAccess
restricted use
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