| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Molecular and cellular characterization of apoptosis in flat oyster a key mechanisms at the heart of host-parasite interactions
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| Autores: |
Gervais, Ophelie
Renault, Tristan
Arzul, Isabelle
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| Data: |
2018-08
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| Ano: |
2018
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| Resumo: |
Bonamia ostreae has been associated with the decline of flat oyster Ostrea edulis populations in some European countries. This obligatory intracellular parasite persists and multiplies into hemocytes. Previous in vitro experiments showed that apoptosis is activated in hemocytes between 1 h and 4 h of contact with the parasite. The flat oyster uses the apoptosis pathway to defend against B. ostreae. However, the parasite might be also able to modulate this response in order to survive in its host. In order to investigate this hypothesis the apoptotic response of the host was evaluated using flow cytometry, transmission electron microscopy and by measuring the response of genes involved in the apoptotic pathway after 4 h. In parallel, the parasite response was investigated by measuring the expression of B. ostreae genes involved in different biological functions including cell cycle and cell death. Obtained results allow describing molecular apoptotic pathways in O. edulis and confirm that apoptosis is early activated in hemocytes after a contact with B. ostreae. Interestingly, at cellular and molecular levels this process appeared downregulated after 44 h of contact. Concurrently, parasite gene expression appeared reduced suggesting that the parasite could inhibit its own metabolism to escape the immune response.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00454/56540/58246.pdf
http://archimer.ifremer.fr/doc/00454/56540/58247.xlsx
http://archimer.ifremer.fr/doc/00454/56540/58248.pdf
DOI:10.1038/s41598-018-29776-x
http://archimer.ifremer.fr/doc/00454/56540/
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| Editor: |
Nature Publishing Group
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| Formato: |
application/pdf
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| Fonte: |
Scientific Reports (2045-2322) (Nature Publishing Group), 2018-08 , Vol. 8 , N. 1 , P. 12494 (12p.)
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| Direitos: |
The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the...
info:eu-repo/semantics/openAccess
restricted use
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