| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Restoration of the di-myo-inositol-phosphate pathway in the piezo-hyperthermophilic archaeon Thermococcus barophilus
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| Autores: |
Cario, Anais
Mizgier, Alex
Thiel, Axel
Jebbar, Mohamed
Oger, Phil M.
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| Data: |
2015-11
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| Ano: |
2015
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| Palavras-chave: |
High hydrostatic pressure
Heat stress
Di-myo-inositol-phosphate
Thermococcus barophilus
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| Resumo: |
Most Thermococcales accumulate di-myo-inositol-phosphate (DIP) as an organic solute as a response to heat stress. We have studied the accumulation of this osmolyte in the high-hydrostatic pressure adapted hyperthermophile Thermococcus barophilus. We found no accumulation of DIP under any of the stress conditions tested, although this archaeon harbors the 3 DIP synthesis genes. Lack of synthesis is due to the lack of expression of TERMP_01135 coding for the second step of DIP synthesis. In contrast to other species, the T. barophilus synthesis operon is interrupted by a four gene locus, in reverse orientation. Restoring an operon like structure at the DIP locus restored DIP synthesis, but did not have an impact on growth characteristics, suggesting that other mechanisms have evolved in this organism to cope with heat stress.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00270/38113/37216.pdf
DOI:10.1016/j.biochi.2015.05.011
http://archimer.ifremer.fr/doc/00270/38113/
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| Editor: |
Elsevier France-editions Scientifiques Medicales Elsevier
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| Formato: |
application/pdf
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| Fonte: |
Biochimie (0300-9084) (Elsevier France-editions Scientifiques Medicales Elsevier), 2015-11 , Vol. 118 , P. 286-293
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| Direitos: |
2015 Elsevier B.V. and Societe Française de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved
info:eu-repo/semantics/openAccess
restricted use
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